Furthermore, stimulation of purified CD4+ and CD8+ T-cells with TLR ligands, specifically TLR-2 (Pam3Csk4), TLR-9 (oligodeoxynucleotide [ODN]), and TLR-4 (LPS), directly triggered cytokine production in the patients with MS. This evidence concerns the gene CD4 and myeloid sarcoma.