We sought to visually depict the intricate relationship between pulmonary fibrosis and lung macrophages, particularly the M2 subtype, by using a multi-fluorescence immunohistochemical staining approach targeting CD206, F4/80, and collagen I. Our findings revealed substantial accumulation of collagen I surrounding the trachea and interstitial spaces in the model group, accompanied by macrophage infiltration and elevated CD206 expression. Here, MRC1 is linked to pulmonary fibrosis.