The intratumoral (i.t.)injection of poly(I:C)+R848-prot-NCs, showed potent reduction in tumor volume and weight compared with control mice, while the poly(I:C)-prot-NCs or the R848-prot-NCs (loaded with only one TLR agonist) presented much lower antitumoral activity (Figures 3A, B). This evidence concerns the gene SLC6A7 and neoplasm.