However, the therapeutic effect of SSB2 on depression is blocked when GPX4 is knocked out, indicating that SSB2 acts through a GPX4-dependent manner in mediating TLR4/NF-κB pathway, exerting its anti-ferroptosis and anti-neuroinflammatory roles (WANG et al., 2023b). This evidence concerns the gene NFKB1 and major depressive disorder.