Anti-biotin-linked CD133/1-biotin magnetic beads (CD133 MicroBead Kit-Tumor Tissue, 130-100-857, Miltenyi, Auburn, CA, USA), LS Columns (Miltenyi, Auburn, CA, USA) for the differentiation of stem cells carrying CD133+ protein, a cancer stem cell surface marker from the SAOS-2 cell line (130-042-401), and LD Columns (130-042-901, Miltenyi, Auburn, CA, USA) were used. The gene discussed is PROM1; the disease is cancer.