Or work suggests that MSTRG.81401 may participate in the pathogenesis of comorbid DNP and MDD by upregulating the expression of P2X7 and activation of hippocampal astrocytes, since shRNA of MSTRG.81401 can reverse these changes, as demonstrated by the significant relief of elevated co-expression of P2X7 and GFAP in rats with comorbid DNP and MDD. This evidence concerns the gene P2RX7 and major depressive disorder.