As for transcriptional regulators validation, we observed that the expression of five of the identified TFs (ETS1, TFAP2A, NFKB2, CTCF, and RELA) is significantly altered in the blood samples of depression patients (datasets GSE217811 and GSE23848) (Table 3); of note, except for ETS1, all transcription factors show increased expression in patients vs. controls. This evidence concerns the gene CTCF and major depressive disorder.