Pathway enrichment analysis using IPA [27] and ShinyGo [28] revealed that genes that were downregulated 24 h post 1 and 6Gy IR (cluster 2) were enriched for cancer-promoting pathways, including the TGF-β signaling pathway, which promotes cell growth and metastasis in the U251 cells [29]. The gene discussed is TGFB1; the disease is cancer.