MCF-7 BC cells exposed to an RPM for five d revealed a downregulation in ACTB, TUBB, EZR, RDX, FN1, VEGFA, FLK1, CASP9, CASP3, and PRKCA mRNAs indicating the involvement of the cytoskeleton, extracellular matrix proteins, caspases, growth factors, and protein kinases in spheroid formation [95]. This evidence concerns the gene ACTB and breast cancer.