MCF-7 BC cells exposed to an RPM for five d revealed a downregulation in ACTB, TUBB, EZR, RDX, FN1, VEGFA, FLK1, CASP9, CASP3, and PRKCA mRNAs indicating the involvement of the cytoskeleton, extracellular matrix proteins, caspases, growth factors, and protein kinases in spheroid formation [95]. The gene discussed is CASP9; the disease is breast cancer.