We examined the effects of Tjp1 and Tjp2 KO expression on tumor growth and metastasis by subcutaneously and intravenously injecting separate groups of mice (10 per test group) with MOCK, Tjp1 and Tjp2 KO, or Tjp1 and Tjp2 re-expressed B16-F10 melanoma cells. This evidence concerns the gene TJP1 and neoplasm.