We focused our studies by targeting molecules in the pediatric brainstem DMG (pbDMG) tumor microenvironment (TME), especially because TGFB ligands play a pivotal role in generating tumors by promoting immune suppression, immune evasion, and tumor progression, which involve inhibiting CD8-antigen-positive cytotoxic T-cells and natural killer cells and activating regulatory T-cells [21,22,23,24,25,26,27,28]. The gene discussed is CD8A; the disease is neoplasm.