Also, both Analysis1 and Analysis3 showed similar results from pathway analysis and enrichment analysis; an increase in activated Akt and FOXO1 pathways was identified as the main predicted pathways, and cancer, reproductive disease, and neuro/psychological diseases were identified as associated diseases with DE-miRNAs and their target genes (Table 1, Supplementary Figures S2 and S3). Here, AKT1 is linked to cancer.