We applied an in vitro cell model to simulate hyperglycemia and found that Cana achieves a direct anti-inflammatory effect in BV-2 microglia exposed to HG by increasing the expression of Bcl-2 and p62 and decreasing the levels of LC3B-II, cleaved caspase-3, activated p53 and proinflammatory factors (i.e., NO, ROS, iNOS, NLRP3, IL-1β and TNF-α). Here, TP53 is linked to Hyperglycemia.