Firstly, among all 153 leading-edge genes, a total of 33 leading-edge genes (ACOXL, CRAT, SESN2, ABCD1, HSD17B10, ECH1, ECHS1, ETFB, ABCB11, SORT1, C1QTNF12, PEA15, SLC27A4, INSR, PC, IDH3G, IDH3B, SDHB, ACO2, SUCLG1, PGM1, HK1, ALDOC, ALDH2, PFKL, ENO1, PFKP, AKR1A1, CYC1, SDHA, NUP210, PPP2R1A, and HK2) were significantly associated with the OS of AML patients (p < 0.05) by univariate Cox regression analysis. The gene discussed is ENO1; the disease is acute myeloid leukemia.