Firstly, among all 153 leading-edge genes, a total of 33 leading-edge genes (ACOXL, CRAT, SESN2, ABCD1, HSD17B10, ECH1, ECHS1, ETFB, ABCB11, SORT1, C1QTNF12, PEA15, SLC27A4, INSR, PC, IDH3G, IDH3B, SDHB, ACO2, SUCLG1, PGM1, HK1, ALDOC, ALDH2, PFKL, ENO1, PFKP, AKR1A1, CYC1, SDHA, NUP210, PPP2R1A, and HK2) were significantly associated with the OS of AML patients (p < 0.05) by univariate Cox regression analysis. This evidence concerns the gene HK1 and acute myeloid leukemia.