Stroke susceptibility of Notch3 knockout mice was rescued by VSMC-specific expression of the p.Arg1031Cys (R1031C) transgene but not rescued by the p.Cys455Arg (C455R) transgene, indicating decreased Notch signaling of p.Cys455Arg in vivo [75] Recently, Hack et al. investigated the NOTCH3 signaling activity of mutant NOTCH3 using NIH 3T3 cells. This evidence concerns the gene NOTCH3 and stroke disorder.