As shown in Fig. 4B, after removing CD8+ T cells, Foxp3+ Tregs, LAMP3+ DCs, and PD-1+ cells, the Mahalanobis distance for different tumor regions (no. 8) was significantly narrowed, indicating that CD8+ T cells, Foxp3+ Tregs, LAMP3+ DCs, and PD-1+ cells were the main influencing factors leading to the TIME heterogeneity in different tumor regions (no. 8). This evidence concerns the gene FOXP3 and neoplasm.