In this study, we isolated serum EVs and cortex brain-derived EVs (BDEVs) from TDP43*Q331K and TDP-43*WT mice, at 3-months-old and 6-months-old, which represented the early-symptomatic stage and the stage which corresponds with prominent motor neuron degeneration [25, 26]. The gene discussed is TARDBP; the disease is Motor neuron atrophy.