Figure 3D shows that the five colonies from NIH3T3 exposed to SW480 cell supernatant (positive control), the five colonies from the SF, and the five colonies from the reconstituted fraction were positive for KRAS. These five colonies from each condition were mixed and expanded in vitro and then injected into mice to investigate the ability to form tumours in immunodeficient mice. The gene discussed is KRAS; the disease is neoplasm.