Consistent with the IPA canonical pathway analysis, the phosphorylation level of 27 proteins (including the three kinases (PDPK1, PAK1, and PTK2B) auto-phosphorylating themselves) were elevated by the three kinases to regulate cardiac stress signaling pathways, including PKA signaling, ERK/MAPK signaling, cardiac hypertrophy signaling (enhanced), and renin-angiotensin signaling (Figure 1E and Figure 1—figure supplement 2), to cope with the cardiac stress in the TGAC8. Here, PAK1 is linked to cardiac hypertrophy.