CD40LG and brucellosis: The current study determined that IgG ELISA plus BrucellaCapt had 8.7% seropositivity, which supported the evaluation of human brucellosis in an endemic area by six serological tests (RBT, standard serum agglutination (SAT), Brucella Coombs, BrucellaCapt, IgM ELISA, and IgG ELISA) that found all tests valuable for positive results, but only Brucella Coombs and BrucellaCapt were reliable for negative serological results [57].