As shown in Figure 8B,E, compared with the other groups, the ratio of P-AKT-Ser473 to total AKT was significantly increased in the insulin overdose group (p < 0.0001 VS control group, p < 0.0001 VS recover group), while the ratio of P-AKT-Thr308 to the total AKT was not significantly changed in the hypoglycemia group (p > 0.05). Here, INS is linked to Hypoglycemia.