Lastly, we showed an IL4I1-mediated cross-talk mechanism between TAMs and glioma cells, as well as its role in macrophage polarization, through a combination of diverse cellular and molecular experiments: co-culture, Western blotting (WB), quantitative real-time PCR (RT-qPCR), immunohistochemistry (IHC), immunofluorescence (IF), enzyme-linked immunosorbent assay (ELISA), and siRNA transfection. This evidence concerns the gene IL4I1 and glioma.