NR3C1 and Anxiety: Animal experiments have also demonstrated that the methylation status of the NR3C1 gene in offspring is sensitive to prenatal stress, prenatal stress is associated with demethylation of the corticotropin-releasing hormone (CRH) promoter, leading to anxiety-like behavior during adolescence and heightened reactivity of the HPA axis (Xu et al., 2014; Öztürk et al., 2022).