In this context, in the present study, we analyzed the anti-glioma effects of rutin on viability, miRNA-125b expression, and STAT3 expression in human GBM cells, as well as its immunomodulatory property during indirect interaction (via secretome) with human microglia, relating inflammatory mediators and modulating STAT3 signaling. The gene discussed is STAT3; the disease is central nervous system cancer.