Certainly, in the natural infection cycle, mixture of virions and EV populations are released and interact with host cells; however, to assess the role of Mø exo ZIKV in viral transmission, we purified the exosomes by inactivation of ZIKV by UV radiation and RNase A treatment (Mø exo ZIKV [RNase A + UV]) or by uncoupling from anti-CD63-coated paramagnetic beads (Mø exo ZIKVp). This evidence concerns the gene CD63 and infection.