In addition, the amounts of GzmB detected in the cultures of the immunized mouse CD8+ T cells with MC38 CRC cells were 4.8 times greater than those in the cultures of the unimmunized mouse T cells with the tumor cells (p < 0.001, Figure 4A), although the GzmB levels in the cultures of the latter T cells were significantly increased when the tumor cells were present in their cultures (p < 0.05, Figure 4A). This evidence concerns the gene CD8A and colorectal carcinoma.