As shown in Supplementary Tables S3, there were no statistically significant associations between the positive rates of serum IGFBP-1 and depth of tumor invasion, lymph node metastasis, distant metastasis, and early-stage or advanced-stage of CRC, but high IGFBP-1 levels were associated with age (P < 0.05) and gender (P < 0.05). This evidence concerns the gene IGFBP1 and metastatic malignant neoplasm in the lymph nodes.