Cell cycle predictions from scRNA-seq analysis of CML and normal CD34+CD38− cells isolated from peripheral blood showed LSC had a noticeably higher proportion of cells in S or G/2 M when compared to HSC (Supplementary Fig. 2a, b) – consistent with cell cycle profiles observed in ESC29. The gene discussed is CD34; the disease is chronic myelogenous leukemia, BCR-ABL1 positive.