GPER1 and fatty liver disease: To further evaluate whether GPER1 also acts a crucial role in the progression of male NASH, the male mice were fed with an HFHC diet for 16 weeks to establish a NASH male model, and we found that GPER1-HKO male mice exhibited more severe hepatic steatosis, inflammation, and fibrosis than that of the GPER1-Flox control male mice (Fig. S7).