The beneficial role of G1 on the progression of NASH was dispelled in hepatic GPER1-KO female mice, which indicated by no significant differences in hepatic steatosis, inflammation, fibrosis, and liver injury were observed between vehicle and G1-treated GPER1-HKO female mice after HFHC consumption (Fig. 5). This evidence concerns the gene GPER1 and Hepatic steatosis.