GPER1 and fatty liver disease: In line with lipid accumulation, H&E and Oil red O staining results also indicated that hepatic GPER1 deletion led to more severe hepatic steatosis than that of the GPER1-Flox control female mice, accompanied by memorably enhanced mRNA levels of genes related to the uptake, transport, and synthesis of fatty acids and remarkably reduced expression levels of genes participating in fatty acid β-oxidation (Figs. 3, C and D and S6K).