Notably, our validation analysis in independent AECs datasets replicated 29 out of the 35 differentially methylated CpGs associated with asthma in BECs and 17 of the differentially methylated CpGs were significantly correlated with expression of annotated DEGs including SERPINE1, SLC9A3, CD9, HNF1A, TINAGL1, and FLNC. Two CpG sites that were methylated across two studies are annotated to hypo-methylated SERPINE1 and SLC9A3. Pampuch et al. showed that SERPINE1 was associated with IgE response and bronchial activity [25]. Here, SERPINE1 is linked to asthma.