LIF and Cachexia: To investigate whether systemic elevation of LIF induces cachexia in mice, we generated a transgenic LIF knock-in mouse model (LIF-tgflox/+; referred to as TgL hereafter) by knocking in the mouse LIF gene, preceded by the CAG promoter and a transcriptional STOP cassette, into the Rosa26 locus (CAG-STOP-LIF-eGFP-Rosa26TV) using the CRISPR/Cas9 system, as described previously15,16 (Fig. 1A).