To demonstrate the potential in situ association between Bmal1 and Myh9, proximity ligation assay (PLA) was performed, revealing that endogenous or ectopically expressed Bmal1 proteins are proximal to Myh9, mostly in the nucleus of mouse YUMM2.1 and several human melanoma cell lines (Fig. 4f, g and Supplementary Fig. 4b,c). This evidence concerns the gene BMAL1 and melanoma.