We assessed the interplay between CAF-associated α-SMA and PROX1, and the impact of these interactions on tumor aggression by coculturing PROX1-expressing CRC cells (SW-480, SW-620, HCT116, and HT-29 cells) with α-SMA-rich CAFs (Figure 5A, 5B). This evidence concerns the gene PROX1 and neoplasm.