NGS identified the TP53 (NM_000546.5):c.708C > A variant in both the liver EAML and pancreatic cancer, with a variant allele frequency (VAF) of 53.05% and 51.63%, respectively, and this was identical to the patient’s germline mutation (Fig. 5C, D). This evidence concerns the gene TP53 and pancreatic neoplasm.