Using a rabbit monoclonal anti-MSH3 antibody (clone 405) we detected nuclear MSH3 protein expression in the non-patient control normal crypts and CRC tumors by IHC, whereas this patient’ MSH3 variant proteins were detected in the cytoplasm and/or cell membrane but not in the nucleus of either the patient’s tumor or normal colon tissues (Fig. 2B). Here, MSH3 is linked to neoplasm.