Therefore, we validated the expression of CHEK2 in five HCC cell lines (97H, LM3, BEL-7404, Huh7 and hepG2) and observed relatively higher expression of CHEK2 in LM3, Huh7, and HepG2 cell lines (sup Fig. 2A). The gene discussed is CHEK2; the disease is hepatocellular carcinoma.