When co-cultured with the E.G7-OVA cells, in vitro differentiated OT-I T cells with CUL5 KO showed significantly higher tumor cell killing ability at several non-saturated effector to target ratios than the NC cells (Fig. 3g), accompanied with significantly increased GZMB and IFNg expression levels upon E.G7-OVA cell stimulation over those of the NC cells (Fig. 3h and Supplementary Fig. 3m). This evidence concerns the gene IFNG and neoplasm.