For instance, scRNA-seq was performed on three melanoma cell lines of different genotypes (BRAF-mutant, NRAS-mutant and BRAF/NRAS-wildtype), and while these genotypes formed distinct cell clusters, additional subclusters were identified within each genotype, comprising divergent proliferative, cell cycle, pigmentation or stromal cell gene signatures.50 This evidence concerns the gene BRAF and melanoma.