In addition, due to the insertion of 2 extra amino acids, the original codon 600 (600V) in the A-loop of the kinase domain were converted into R, activating the regulation of signal transduction from RAS to MEK inside the cell.[26] Meanwhile, combined with the results of cytopathology, this nonclassical variant of BRAF might be associated with the malignancy of thyroid nodule and contribute to thyroid carcinomas. This evidence concerns the gene BRAF and thyroid gland carcinoma.