Test for a possible regulatory role of miR-194 in the production of cytokines IL-1β, IL-6, IL-4, TNF-α, IFN-y, TGF-β, and IL-10, PBMCs from healthy dogs and those with leishmaniasis were transfected with miR-194 Mimic and Inhibitor. Here, TGFB1 is linked to leishmaniasis.