No changes were observed in the production of IL-6 (Fig 5C and 5D), TNF-α (Fig 5E and 5F), or IFN-y (Fig 5G and 5H) in the PBMC culture supernatants of healthy dogs and those with leishmaniasis after transfection with miR-194 Mimic and Inhibitor. This evidence concerns the gene TNF and leishmaniasis.