To further determine whether the reduced expression of Rbfox1 in DRG was sufficient to trigger neuropathic pain following peripheral nerve injury, we mimicked the peripheral nerve injury-induced reduction in DRG Rbfox1 by microinjection of AAV5-Rbfox1 shRNA into the ipsilateral L3 and L4 DRG of naive mice. This evidence concerns the gene RBFOX1 and peripheral nerve injury.