A fluorescence dual MM-based aptassay for simultaneous, fast, and accurate determination of highly relevant biomarkers such as PCT and IL-6 in clinical samples coming from neonates with gestational age less than 32 weeks and birthweight below 1000 g with suspected (late-onset) sepsis, has successfully been developed, making MM technology a promising and competitive tool in diagnosing of this relevant disease. Here, IL6 is linked to Sepsis.