There was no difference in the amount of GFAP immunoreactivity 24 h post-stroke between the TKO and WT mice (p = 0.104, Figure 2B), however TKO mice had significantly reduced GFAP levels compared to the WT mice 7 days post-stroke (p = 0.011) and 30 days post-stroke (p < 0.0001; Figures 2D, F). Here, GFAP is linked to stroke disorder.