Cluster 5 high expression genes include Apoe, Cd63, Cxcl2, Cd52, Ctsb, H2-D1, Fth1 and Spp1 (Fig. 4D), and cluster 5 has 22 downregulated and 46 upregulated genes that are similar to disease-associated microglia (DAM) and ALS (Fig. 4E and F). This evidence concerns the gene SPP1 and amyotrophic lateral sclerosis.