HCN4 and neuroblastoma: Notably, this pathway encompassed a set of significantly altered genes including CGA, ADRB1, GIP, ADCY1, SST, FFAR2, HCN4, PPP1R1B, PTCH1, HHIP, LIPE, TNNI3, PLN, FXYD1, GRIA1, and GRIN3A. As depicted in Figure 5C, the PI3K-Akt signaling pathway exhibited alterations in the expression levels of CSF1, CSF1R, PCK1, IL6, CHAD and TCL1A between HG-NB and LG-NB.