To identify sequences of TCRs recognizing neo-epitopes by single RNA cell sequencing, epitopes from shared tumor-associated antigens as well as viral epitopes, CD8+ T cells from the peripheral blood of the melanoma patient were labeled with pools of peptide-loaded MHC-I molecules multimerized with DNA-barcoded PE-conjugated dCODE Dextramer® reagents (Figure 7B, Supplementary Figure 2C, Supplementary Table 4). Here, CD8A is linked to melanoma.