Enabled by DNA-barcoded pMHC-I multimers representing a combination of peptide-loaded dsSCDs, easYmers and peptide-tethered dtSCTs, we successfully conducted scRNA-Seq of the melanoma patient-derived pMHC-I multimer+ CD8+ T cell population leading to the discovery of novel MAGE-A1/A*01:01 and NY-ESO-1/C*08:02 T cell receptors that could be functionally validated by recombinant expression in Jurkat reporter cells and that could be useful for other patients sharing these HLA-I alleles. The gene discussed is MAGEA1; the disease is melanoma.