By staining CD8+ T cells from PBMC using multimerized easYmers and dsSCD in a complementary manner that were color-coded with streptavidin-fluorochrome conjugates in a 60-fold matrix, we detected 3 HLA-B*08:01- and HLA-C*08:02-restricted T cell populations reactive with tumor-specific point mutations and a neo-sequence resulting from an out-of-frame gene fusion event. This evidence concerns the gene CD8A and neoplasm.