HLA-A and melanoma: Next, we set out to apply the MediMer peptide binding assay to the six HLA-A,B,C allotypes and 166 unique tumor neoepitope peptides predicted by NetMHCpan 4.1 (61) after whole exome sequencing of tumor cells from a lymph node metastasis of an advanced-stage melanoma patient (Supplementary Table 3).