NOTCH1 and neoplasm: However, examination of each of the clusters for EMT marker expression in lung mets vs GPx2KD primary tumour, revealed upregulation of luminal (Cdh1, Epcam, Cldn3/7), basal/mesenchymal (Krt17, Vim, Snai1/2, Twist1) and (Sox4, Jag1, Notch1) genes in lung mets clusters, which was especially striking in mesenchymal (M) cluster 2 (Fig. 3f).