The distal tubule injury marker NGAL showed a trend towards upregulation in both pregnant Wistar Kyoto rats and Stroke-Prone Spontaneously Hypertensive rats (FC: 1.3, p 0.06) compared to non-pregnant rats, however, there was no difference in expression between pregnant Wistar Kyoto rats and pregnant Stroke-Prone Spontaneously Hypertensive rats (Fig. 2e). This evidence concerns the gene LCN2 and stroke disorder.