To directly clarify whether LIPUS alleviates myocardial inflammation and CD4+ T-cell differentiation in ICI-related myocarditis by activating the HIPPO pathway, the HIPPO inhibitor XMU-MP-1 was used to constrain the scaffold protein Mst1, followed by LIPUS treatment after the preparation of the ICI-related myocarditis model. This evidence concerns the gene CD4 and myocarditis.