To comprehensively dissect the impact of NLRC5 overexpression in the TME on T and NK lymphocytes present in both the tumor and ascites, we further examined their functional characteristics by assessing the expression of activation/exhaustion markers (CD127, CD25, Ly6C, PD-1, KLRG1, LAG3, CD69, NKG2D) as summarized in Supplementary Figure 5A. The gene discussed is LAG3; the disease is neoplasm.